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Codex Biosolutions hek-cng+cb2
(A) Functional activities of SMM-295 in the <t>CB2</t> ACTOne assay (open circles), CB1 ACTOne assay (open triangles), and parental ACTOne cells containing only the <t>CNG</t> ion channel (filled squares). The functional activation of CB2 by the internal control (CP 55,940; filled diamonds) is shown. (B) Western blot analysis of signaling proteins after brief exposure (15 minutes) to SMM-295 in rat NRK-52E proximal tubule epithelial cells. Activation of prosurvival Akt/PKB and MAPK (ERK1/2 and p38) was detected using specific antibodies. β-actin was used as a loading control. ERK1/2, extracellular signal–regulated kinase 1/2.
Hek Cng+Cb2, supplied by Codex Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Functional activities of SMM-295 in the CB2 ACTOne assay (open circles), CB1 ACTOne assay (open triangles), and parental ACTOne cells containing only the CNG ion channel (filled squares). The functional activation of CB2 by the internal control (CP 55,940; filled diamonds) is shown. (B) Western blot analysis of signaling proteins after brief exposure (15 minutes) to SMM-295 in rat NRK-52E proximal tubule epithelial cells. Activation of prosurvival Akt/PKB and MAPK (ERK1/2 and p38) was detected using specific antibodies. β-actin was used as a loading control. ERK1/2, extracellular signal–regulated kinase 1/2.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Selective Cannabinoid 2 Receptor Stimulation Reduces Tubular Epithelial Cell Damage after Renal Ischemia-Reperfusion Injury

doi: 10.1124/jpet.117.245522

Figure Lengend Snippet: (A) Functional activities of SMM-295 in the CB2 ACTOne assay (open circles), CB1 ACTOne assay (open triangles), and parental ACTOne cells containing only the CNG ion channel (filled squares). The functional activation of CB2 by the internal control (CP 55,940; filled diamonds) is shown. (B) Western blot analysis of signaling proteins after brief exposure (15 minutes) to SMM-295 in rat NRK-52E proximal tubule epithelial cells. Activation of prosurvival Akt/PKB and MAPK (ERK1/2 and p38) was detected using specific antibodies. β-actin was used as a loading control. ERK1/2, extracellular signal–regulated kinase 1/2.

Article Snippet: Human embryonic kidney (HEK)–cyclic nucleotide gated (CNG), HEK-CNG+CB1, and HEK-CNG+CB2 cells were obtained from Codex BioSolutions.

Techniques: Functional Assay, Activation Assay, Western Blot